Nickel-Nta-Nanogold Binds his-Tagged Proteins
نویسندگان
چکیده
منابع مشابه
Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins.
Hollow nickel silicate nanospheres (NiSiO3 NSs) with hierarchical shells were hydrothermally synthesized by using silica spheres as a template. The NiSiO3 NSs have an average diameter of 250 nm with a shell thickness of 50 nm, and the hierarchical shell consists of a large number of sheets. By taking advantage of the high affinity of Ni(2+) toward histidine-tagged (His-tagged) proteins, hollow ...
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Small molecule-based fluorescent probes have been used for real-time visualization of live cells and tracking of various cellular events with minimal perturbation on the cells being investigated. Given the wide utility of the (histidine)6-Ni(2+)-nitrilotriacetate (Ni-NTA) system in protein purification, there is significant interest in fluorescent Ni(2+)-NTA-based probes. Unfortunately, previou...
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While BIACORE instruments are routinely used for kinetic measurements and for the determination of binding constants, the immobilization of a ligand onto the sensor chip surface has to be individually optimized for every system. We show here that the histidine (His) tag, routinely used in protein purification and in detection is an ideal tag for immobilization, despite the intrinsically low aff...
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Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled prot...
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A tag comprising four to six histidines genetically fused to the protein of interest (His-tag) has been widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). Here we report the utilization of the same tag twice in series, first for IMAC and subsequently for immunoaffinity purification. Both steps are based on completely different physical principles and can the...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2002
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927602102510